to set up the software for your use. This guide shows a workflow that uses application settings. BD FACSDiva Software Quick Reference Guide for the BD LSR II. Compensation Controls in the BD FACSDiva Software Reference Manual. • Default templates are now provided for certain instrument functions. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then Open the BD FACSDiva software and log on. Allow the.

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When a parameter name includes a hyphen indicating it is a fluorophore conjugate and a label has already been assigned in Experiment Layout or the Inspector, BD FACSDiva software automatically assigns the label to the corresponding compensation control.

By default, exported configurations are saved in D: When the batch is finished, a message is displayed. Tip To further subset a population that has no color assigned to ityou must first change the color. After events have been recorded, the pointer reverts to green and a disk icon is added to the tube. Browser Functions The following functions have been added to improve the experiment Browser.

Provides shortcuts for softwware database elements, accessing elementspecific options, and acquiring and recording data. The T cells now change to orange in all plots. Resize a line on the worksheet by dragging one of the black handles on either end of the selected line; move the line by selecting the line and dragging. Use the PDF Filename field to designate the filename; use its Browse button to choose where to save the file.

For example, note how the two Paste options affect the data for the following compensation tubes. This chapter contains information about instrument and acquisition controls that are common to all facsdivs.


Menus and Keyboard Shortcuts Software Menus. Figure Browser with representative experiments Browser toolbar search field column divider column header user icon open experiment current tube pointer closed experiment Users with administrative access can view all experiments in the database.


Tip When your experiment contains many tubes that share the same parameter labels, use Experiment Layout to enter all labels at once. When you append data to a recorded tube, time is added to the existing data set.

Template names cannot include periods or commas. Simpler Gating Features Proportional resizing of polygon and rectangular gates. Once a user has experiments, you can either disable the user name or delete their experiments and then delete the user name. Tip Multiple worksheet objects can be selected for export.

The second example organizes QC work by month at the experiment level, by sample type at the specimen level, refernece by date at the tube level. Select button—selects by clickingmoves by draggingor resizes by dragging a selection handle of objects manuak a worksheet.

Report for optimized settings. To move objects between worksheets, use the copy and paste functions; objects cannot be dragged. The gate boundary is resized proportionally according to the original shape.

Note that panel names cannot include periods or commas. This guide assumes that you have a user account for your Remote Access service. Administrators can access the latest user tracking log file through the File menu. To rename a template category, rename the softwsre the template is stored in. For either manhal, set the maximum value or have it automatically calculated by the software. The Analysis Templates dialog box appears where you can choose an analysis template to import.

BD FACSCanto II Quick Reference Manual

Unlike an autointerval gate, a snap-to interval gate automatically recalculates as new data is provided to the gate. Summary of the Upgrade Process on page 6. For an example showing each scale method, see the following figure.

Area Scaling Sample Plot 0. Getting Connected More information. If the file you are importing contains a duplicate of any existing user names, the following message appears: No part of this publication may be reproduced, transmitted, transcribed, stored in retrieval systems, or translated into any language or computer language, in any form or by any means: The information contained herein.


If the window is too narrow, pulses might be measured incompletely. Click OK to dismiss the dialog box.

BD FACSDiva Software Reference Manual

Dell Active Administrator 7. BD Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments. When fluidics shutdown completes, turn off the power.

Experiments are deleted from the Browser after export. After login, other users cannot view your experiments unless they have administrative access, or you have designated an experiment as shared.

BD FACSDiva Software Reference Manual – PDF

InfoPrint Serial Matrix Printers. The length of the x- and y-axes halves each time you use the button.

Tools for Data Analysis Using the Worksheet Inspector Use the Worksheet Inspector to name a worksheet or global worksheet, increase the number of pages in the worksheet, and hide or show page breaks and page numbers. See Undo and Redo Functions on page Tip You can also drag and drop the gate by pressing the Ctrl key then clicking and dragging the gate.

Once instrument settings have been linked to a setup, you cannot edit compensation values manually. Don’t show me this message again. Gating Multicolor Experiments The following is an advanced gating tutorial that demonstrates derived, interval, and quadrant gating features through the analysis of lymphocyte subsets in a human peripheral blood sample. You can work referenc only one worksheet, the active worksheet, at a time.